Non‐radioactive analysis of lipid‐linked oligosaccharide compositions by fluorophore‐assisted carbohydrate electrophoresis

N Gao, MA Lehrman - Methods in enzymology, 2006 - Elsevier
Lipid‐linked oligosaccharides (LLOs) are the donors of glycans that modify newly
synthesized proteins in the endoplasmic reticulum (ER) of eukaryotes, resulting in formation
of N‐linked glycoproteins. The vast majority of LLO analyses have relied on metabolic
labeling with radioactive sugar precursors, but these approaches have technical limitations
resulting in many important questions about LLO synthesis being left unanswered. Here we
describe the application of a facile non‐radioactive technique, fluorophore‐assisted …