The use of fluorescence quenching in flow cytofluorometry to measure the attachment and ingestion phases in phagocytosis in peripheral blood without prior cell …

J Hed, G Hallden, SGO Johansson… - Journal of immunological …, 1987 - Elsevier
Flow cytofluorimetry identifies and quantifies cell markers of different leukocyte
subpopulations by combining cytofluorimetry with the differences in the light scattering
properties of the leukocytes in mixed populations. In the phagocytic assay, reported in this
paper, the experimental conditions were selected in such a way that it was possible to
analyse the phagocytic function of granulocytes in peripheral blood without time-consuming
cell separation. The percentage of phagocytosing granulocytes was not dependent on the …