miR-181 targets multiple Bcl-2 family members and influences apoptosis and mitochondrial function in astrocytes
YB Ouyang, Y Lu, S Yue, RG Giffard - Mitochondrion, 2012 - Elsevier
Mitochondria are central to the execution of apoptosis, and the Bcl-2 protein family of pro-
and anti-apoptotic proteins interacts with mitochondria to regulate apoptosis. Using
bioinformatics we predicted that miR-181, a microRNA expressed in brain, could target the
3′ UTRs of Bcl-2 family members Bcl-2-L11/Bim, Mcl-1, and Bcl-2. Using the luciferase
reporter assay we confirmed these targets. We used mimic and inhibitor to alter miR-181a
levels in primary astrocyte cultures and found miR-181a reduction was associated with …
and anti-apoptotic proteins interacts with mitochondria to regulate apoptosis. Using
bioinformatics we predicted that miR-181, a microRNA expressed in brain, could target the
3′ UTRs of Bcl-2 family members Bcl-2-L11/Bim, Mcl-1, and Bcl-2. Using the luciferase
reporter assay we confirmed these targets. We used mimic and inhibitor to alter miR-181a
levels in primary astrocyte cultures and found miR-181a reduction was associated with …