[HTML][HTML] Identification of a functionally critical protein kinase C phosphorylation residue of cardiac troponin T
MP Sumandea, WG Pyle, T Kobayashi… - Journal of Biological …, 2003 - ASBMB
Cardiac Troponin T (cTnT) is one prominent substrate through which protein kinase C (PKC)
exerts its effect on cardiomyocyte function. To determine the specific functional effects of the
cTnT PKC-dependent phosphorylation sites (Thr 197, Ser 201, Thr 206, and Thr 287) we first
mutated these residues to glutamate (E) or alanine (A). cTnT was selectively mutated to
generate single, double, triple, and quadruple mutants. Bacterially expressed mutants were
evaluated in detergent-treated mouse left ventricular papillary muscle fiber bundles where …
exerts its effect on cardiomyocyte function. To determine the specific functional effects of the
cTnT PKC-dependent phosphorylation sites (Thr 197, Ser 201, Thr 206, and Thr 287) we first
mutated these residues to glutamate (E) or alanine (A). cTnT was selectively mutated to
generate single, double, triple, and quadruple mutants. Bacterially expressed mutants were
evaluated in detergent-treated mouse left ventricular papillary muscle fiber bundles where …