[HTML][HTML] Single cell Ras-GTP analysis reveals altered Ras activity in a subpopulation of neurofibroma Schwann cells but not fibroblasts
LS Sherman, R Atit, T Rosenbaum, AD Cox… - Journal of Biological …, 2000 - ASBMB
Neurofibromatosis type 1 (NF1) is a common genetic disorder characterized by multiple
neurofibromas, peripheral nerve tumors containing mainly Schwann cells and fibroblasts.
The NF1 gene encodes neurofibromin, a tumor suppressor postulated to function in part as a
Ras GTPase-activating protein. The roles of different cell types and of elevated Ras-GTP in
neurofibroma formation are unclear. To determine which neurofibroma cell type has altered
Ras-GTP regulation, we developed an immunocytochemical assay for active, GTP-bound …
neurofibromas, peripheral nerve tumors containing mainly Schwann cells and fibroblasts.
The NF1 gene encodes neurofibromin, a tumor suppressor postulated to function in part as a
Ras GTPase-activating protein. The roles of different cell types and of elevated Ras-GTP in
neurofibroma formation are unclear. To determine which neurofibroma cell type has altered
Ras-GTP regulation, we developed an immunocytochemical assay for active, GTP-bound …