[HTML][HTML] Topologically conserved residues direct heme transport in HRG-1-related proteins
Caenorhabditis elegans and human HRG-1-related proteins are conserved, membrane-
bound permeases that bind and translocate heme in metazoan cells via a currently
uncharacterized mechanism. Here, we show that cellular import of heme by HRG-1-related
proteins from worms and humans requires strategically located amino acids that are
topologically conserved across species. We exploit a heme synthesis-defective
Saccharomyces cerevisiae mutant to model the heme auxotrophy of C. elegans and …
bound permeases that bind and translocate heme in metazoan cells via a currently
uncharacterized mechanism. Here, we show that cellular import of heme by HRG-1-related
proteins from worms and humans requires strategically located amino acids that are
topologically conserved across species. We exploit a heme synthesis-defective
Saccharomyces cerevisiae mutant to model the heme auxotrophy of C. elegans and …