[HTML][HTML] Mutator phenotype of MUTYH-null mouse embryonic stem cells
S Hirano, Y Tominaga, A Ichinoe, Y Ushijima… - Journal of Biological …, 2003 - ASBMB
To evaluate the antimutagenic role of a mammalian mutY homolog, namely the Mutyh gene,
which encodes adenine DNA glycosylase excising adenine misincorporated opposite 8-
oxoguanine in the template DNA, we generated MUTYH-null mouse embryonic stem (ES)
cells. In the MUTYH-null cells carrying no adenine DNA glycosylase activity, the
spontaneous mutation rate increased 2-fold in comparison with wild type cells. The
expression of wild type mMUTYH or mutant mMUTYH protein with amino acid substitutions …
which encodes adenine DNA glycosylase excising adenine misincorporated opposite 8-
oxoguanine in the template DNA, we generated MUTYH-null mouse embryonic stem (ES)
cells. In the MUTYH-null cells carrying no adenine DNA glycosylase activity, the
spontaneous mutation rate increased 2-fold in comparison with wild type cells. The
expression of wild type mMUTYH or mutant mMUTYH protein with amino acid substitutions …