Culturing satellite cells from living single muscle fiber explants
JD Rosenblatt, AI Lunt, DJ Parry… - In Vitro Cellular & …, 1995 - Springer
JD Rosenblatt, AI Lunt, DJ Parry, TA Partridge
In Vitro Cellular & Developmental Biology-Animal, 1995•SpringerConventional methods for isolating myogenic (satellite) cells are inadequate when only
small quantities of muscle, the tissue in which satellite cells reside, are available. We have
developed a tissue culture system that reliably permits isolation of intact, living, single
muscle fibers with associated satellite cells from predominantly fast and slow muscles of rat
and mouse; maintenance of the isolated fibers in vitro; dissociation, proliferation, and
differentiation of satellite cells from each fiber; and removal of the fiber from culture for …
small quantities of muscle, the tissue in which satellite cells reside, are available. We have
developed a tissue culture system that reliably permits isolation of intact, living, single
muscle fibers with associated satellite cells from predominantly fast and slow muscles of rat
and mouse; maintenance of the isolated fibers in vitro; dissociation, proliferation, and
differentiation of satellite cells from each fiber; and removal of the fiber from culture for …
Summary
Conventional methods for isolating myogenic (satellite) cells are inadequate when only small quantities of muscle, the tissue in which satellite cells reside, are available. We have developed a tissue culture system that reliably permits isolation of intact, living, single muscle fibers with associated satellite cells from predominantly fast and slow muscles of rat and mouse; maintenance of the isolated fibers in vitro; dissociation, proliferation, and differentiation of satellite cells from each fiber; and removal of the fiber from culture for analysis.
Springer