Extension of bacteriophage lambda host range: selection, cloning, and characterization of a constitutive lambda receptor gene.

GE de Vries, CK Raymond… - Proceedings of the …, 1984 - National Acad Sciences
GE de Vries, CK Raymond, RA Ludwig
Proceedings of the National Academy of Sciences, 1984National Acad Sciences
A set of plasmids has been constructed that carry a constitutive lamB gene (LamBc
phenotype) from Escherichia coli and that confer functional phage lambda receptors to
bacteria other than E. coli. This E. coli LamBc strain has been selected to escape both
maltose-inducible and glucose-repressible control. Constitutivity results from an IS-3
insertion, carrying a mobile promoter, proximal to lamB. The LamBc DNA has been cloned
into both broad and narrow host-range plasmids, and the resulting pTROY plasmids have …
A set of plasmids has been constructed that carry a constitutive lamB gene (LamBc phenotype) from Escherichia coli and that confer functional phage lambda receptors to bacteria other than E. coli. This E. coli LamBc strain has been selected to escape both maltose-inducible and glucose-repressible control. Constitutivity results from an IS-3 insertion, carrying a mobile promoter, proximal to lamB. The LamBc DNA has been cloned into both broad and narrow host-range plasmids, and the resulting pTROY plasmids have been transferred to diverse bacteria. Both Salmonella typhimurium/pTROY and Klebsiella pneumoniae/pTROY strains efficiently adsorb phage lambda; Pseudomonas aeruginosa/pTROY strains do not. Introduction of a functional E. coli LamB protein into foreign bacterial will allow these bacteria carrying pTROY plasmids to be infected by phage lambda recombinant DNA libraries, phage lambda::Tn insertion mutagenesis vectors, and in vivo lambda-packaged cosmids.
National Acad Sciences