Development of a lethal Shiga toxin-producing Escherichia coli-infection mousemodel using multiple mitomycin C treatment
K Shimizu, T Asahara, K Nomoto, R Tanaka… - Microbial …, 2003 - Elsevier
The aim of this study was to develop a lethal Shiga toxin-producing Escherichia coli (STEC)
infection model in mice. A small inoculum of 5× 103 CFU of STEC strain 89020087 to mice
treated with streptomycin sulfate in drinking water (5 mg/ml) lead to marked increase in the
excretion of the bacteria of up to 109CFU/g feces within 18 h after the challenge.
Combination of administration of 5× 103 CFU of STEC followed by mitomycin C (MMC)
treatment during the late log phase to the early stationary phase of STEC growth in the …
infection model in mice. A small inoculum of 5× 103 CFU of STEC strain 89020087 to mice
treated with streptomycin sulfate in drinking water (5 mg/ml) lead to marked increase in the
excretion of the bacteria of up to 109CFU/g feces within 18 h after the challenge.
Combination of administration of 5× 103 CFU of STEC followed by mitomycin C (MMC)
treatment during the late log phase to the early stationary phase of STEC growth in the …