[HTML][HTML] Cytoskeletal anchoring of GLAST determines susceptibility to brain damage: an identified role for GFAP
Glial fibrillary acidic protein (GFAP) is an enigmatic protein; it currently has no
unambiguously defined role. It is expressed in the cytoskeleton of astrocytes in the
mammalian brain. We have used co-immunoprecipitation to identify in vivo binding partners
for GFAP in the rat and pig brain. We demonstrate interactions between GFAP, the glutamate
transporter GLAST, the PDZ-binding protein NHERF1, and ezrin. These interactions are
physiologically relevant; we demonstrate in vitro that transport of d-aspartate (a glutamate …
unambiguously defined role. It is expressed in the cytoskeleton of astrocytes in the
mammalian brain. We have used co-immunoprecipitation to identify in vivo binding partners
for GFAP in the rat and pig brain. We demonstrate interactions between GFAP, the glutamate
transporter GLAST, the PDZ-binding protein NHERF1, and ezrin. These interactions are
physiologically relevant; we demonstrate in vitro that transport of d-aspartate (a glutamate …