Contrasting roles for STAT4 and STAT6 signal transduction pathways in murine renal ischemia-reperfusion injury

N Yokota, M Burne-Taney… - American Journal of …, 2003 - journals.physiology.org
N Yokota, M Burne-Taney, L Racusen, H Rabb
American Journal of Physiology-Renal Physiology, 2003journals.physiology.org
Recent data support a modulatory role for CD4 T cells in experimental renal ischemia-
reperfusion injury (IRI). CD4 T cells can functionally differentiate to either a Th1 (IFN-γ
producing) or the counterbalancing Th2 (IL-4) phenotype. The enzymes signal transducers
and activators of transcription (STAT) 4 and STAT6 regulate Th1 or Th2 differentiation and
cytokine production, respectively. We therefore hypothesized that mice that were STAT4
deficient would be protected from renal IRI and that STAT6-deficient mice would have a …
Recent data support a modulatory role for CD4 T cells in experimental renal ischemia-reperfusion injury (IRI). CD4 T cells can functionally differentiate to either a Th1 (IFN-γ producing) or the counterbalancing Th2 (IL-4) phenotype. The enzymes signal transducers and activators of transcription (STAT) 4 and STAT6 regulate Th1 or Th2 differentiation and cytokine production, respectively. We therefore hypothesized that mice that were STAT4 deficient would be protected from renal IRI and that STAT6-deficient mice would have a more severe course. Intracellular cytokine staining of splenocytes from STAT4–/– or STAT6–/– exhibited distinct IFN-γ and IL-4 cytokine expression profiles. STAT6–/– had markedly worse renal function and tubular injury postischemia compared with wild type. STAT4–/– had only mildly improved function. Renal phagocyte infiltration and ICAM-1 upregulation were similar in STAT4–/–, STAT6–/–, and wild type. To evaluate if the mechanism of the marked worsening in the STAT6–/– mice could be due to IL-4 deficiency, IL-4-deficient mice were studied and had similar postischemic phenotype to STAT6–/– mice. These data demonstrate that the STAT6 pathway has a major protective role in renal IRI. IL-4 deficiency is a likely mechanism underlying the STAT6 effect. A “yin-yang” role for inflammation is emerging in renal IRI, similar to recent observations in atherosclerosis.
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