Chemical and physiological characterization of fluo-4 Ca2+-indicator dyes
KR Gee, KA Brown, WNU Chen, J Bishop-Stewart… - Cell calcium, 2000 - Elsevier
We have developed fluo-4, a new fluorescent dye for quantifying cellular Ca2+
concentrations in the 100 nM to 1 μM range. Fluo-4 is similar in structure and spectral
properties to the widely used fluorescent Ca2+-indicator dye, fluo-3, but it has certain
advantages over fluo-3. Due to its greater absorption near 488 nm, fluo-4 offers substantially
brighter fluorescence emission when used with excitation by argon-ion laser or other
sources in conjunction with the standard fluorescein filter set. In vitro, fluo-4 exhibited high …
concentrations in the 100 nM to 1 μM range. Fluo-4 is similar in structure and spectral
properties to the widely used fluorescent Ca2+-indicator dye, fluo-3, but it has certain
advantages over fluo-3. Due to its greater absorption near 488 nm, fluo-4 offers substantially
brighter fluorescence emission when used with excitation by argon-ion laser or other
sources in conjunction with the standard fluorescein filter set. In vitro, fluo-4 exhibited high …