[HTML][HTML] Overexpressed GM1 suppresses nerve growth factor (NGF) signals by modulating the intracellular localization of NGF receptors and membrane fluidity in …
M Nishio, S Fukumoto, K Furukawa, A Ichimura… - Journal of Biological …, 2004 - ASBMB
Ganglioside GM1 has been considered to have a neurotrophic factor-like activity. To analyze
the effects of endogenously generated GM1, the rat pheochromocytoma cell line PC12 was
transfected with the GM1/GD1b/GA1 synthase gene and showed increased expression
levels of GM1. To our surprise, GM1+-transfectant cells (GM1+ cells) showed no neurite
formation after stimulation with nerve growth factor (NGF). Autophosphorylation of NGF
receptor TrkA and activation of ERK1/2 after NGF treatment were scarcely detected in GM1+ …
the effects of endogenously generated GM1, the rat pheochromocytoma cell line PC12 was
transfected with the GM1/GD1b/GA1 synthase gene and showed increased expression
levels of GM1. To our surprise, GM1+-transfectant cells (GM1+ cells) showed no neurite
formation after stimulation with nerve growth factor (NGF). Autophosphorylation of NGF
receptor TrkA and activation of ERK1/2 after NGF treatment were scarcely detected in GM1+ …