[HTML][HTML] Rheb inhibits C-raf activity and B-raf/C-raf heterodimerization
M Karbowniczek, GP Robertson, EP Henske - Journal of biological …, 2006 - ASBMB
The Ras-Raf-MEK signaling cascade is critical for normal development and is activated in
many forms of cancer. We have recently shown that B-Raf kinase interacts with and is
inhibited by Rheb, the target of the GTPase-activating domain of the tuberous sclerosis
complex 2 gene product tuberin. Here, we demonstrate for the first time that activation of
Rheb is associated with decreased B-Raf and C-Raf phosphorylation at residues Ser-446
and Ser-338, respectively, concomitant with a decrease in the activities of both kinases and …
many forms of cancer. We have recently shown that B-Raf kinase interacts with and is
inhibited by Rheb, the target of the GTPase-activating domain of the tuberous sclerosis
complex 2 gene product tuberin. Here, we demonstrate for the first time that activation of
Rheb is associated with decreased B-Raf and C-Raf phosphorylation at residues Ser-446
and Ser-338, respectively, concomitant with a decrease in the activities of both kinases and …