Neural stem cells in the adult mammalian forebrain: a relatively quiescent subpopulation of subependymal cells
CM Morshead, BA Reynolds, CG Craig, MW McBurney… - Neuron, 1994 - cell.com
CM Morshead, BA Reynolds, CG Craig, MW McBurney, WA Staines, D Morassutti, S Weiss…
Neuron, 1994•cell.comDissection of the subependyma from the lateral ventricle of the adult mouse forebrain is
necessary and sufficient for the in vitro formation of clonally derived spheres of cells that
exhibit stem cell properties such as selfmaintenance and the generation of a large number
of progeny comprising the major cell types found in the central nervous system. Killing the
constitutively proliferating cells of the subependyma in vivo has no effect on the number of
stem cells isolated in vitro and induces a complete repopulation of the subependyma in vivo …
necessary and sufficient for the in vitro formation of clonally derived spheres of cells that
exhibit stem cell properties such as selfmaintenance and the generation of a large number
of progeny comprising the major cell types found in the central nervous system. Killing the
constitutively proliferating cells of the subependyma in vivo has no effect on the number of
stem cells isolated in vitro and induces a complete repopulation of the subependyma in vivo …
Summary
Dissection of the subependyma from the lateral ventricle of the adult mouse forebrain is necessary and sufficient for the in vitro formation of clonally derived spheres of cells that exhibit stem cell properties such as selfmaintenance and the generation of a large number of progeny comprising the major cell types found in the central nervous system. Killing the constitutively proliferating cells of the subependyma in vivo has no effect on the number of stem cells isolated in vitro and induces a complete repopulation of the subependyma in vivo by relatively quiescent stem cells found within the subependyma. Depleting the relatively quiescent cell population within the subependyma in vivo results in a corresponding decrease in spheres formed in vitro and in the final number of constitutively proliferating cells in vivo, suggesting that a relatively quiescent subependymal cell is the in vivo source of neural stem cells.
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