Marked differences in differentiation propensity among human embryonic stem cell lines
K Osafune, L Caron, M Borowiak, RJ Martinez… - Nature …, 2008 - nature.com
Nature biotechnology, 2008•nature.com
The differentiation potential of 17 human embryonic stem (hES) cell lines was compared.
Some lines exhibit a marked propensity to differentiate into specific lineages, often with> 100-
fold differences in lineage-specific gene expression. For example, HUES 8 is best for
pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial
differences in developmental potential among hES cell lines point to the importance of
screening and deriving lines for lineage-specific differentiation.
Some lines exhibit a marked propensity to differentiate into specific lineages, often with> 100-
fold differences in lineage-specific gene expression. For example, HUES 8 is best for
pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial
differences in developmental potential among hES cell lines point to the importance of
screening and deriving lines for lineage-specific differentiation.
Abstract
The differentiation potential of 17 human embryonic stem (hES) cell lines was compared. Some lines exhibit a marked propensity to differentiate into specific lineages, often with >100-fold differences in lineage-specific gene expression. For example, HUES 8 is best for pancreatic differentiation and HUES 3 for cardiomyocyte generation. These non-trivial differences in developmental potential among hES cell lines point to the importance of screening and deriving lines for lineage-specific differentiation.
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