Derivation of human embryonic stem cells in defined conditions
TE Ludwig, ME Levenstein, JM Jones… - Nature …, 2006 - nature.com
TE Ludwig, ME Levenstein, JM Jones, WT Berggren, ER Mitchen, JL Frane, LJ Crandall…
Nature biotechnology, 2006•nature.comWe have previously reported that high concentrations of basic fibroblast growth factor
(bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those
conditions included poorly defined serum and matrix components. Here we report feeder-
independent human ES cell culture that includes protein components solely derived from
recombinant sources or purified from human material. We describe the derivation of two new
human ES cell lines in these defined culture conditions.
(bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those
conditions included poorly defined serum and matrix components. Here we report feeder-
independent human ES cell culture that includes protein components solely derived from
recombinant sources or purified from human material. We describe the derivation of two new
human ES cell lines in these defined culture conditions.
Abstract
We have previously reported that high concentrations of basic fibroblast growth factor (bFGF) support feeder-independent growth of human embryonic stem (ES) cells, but those conditions included poorly defined serum and matrix components. Here we report feeder-independent human ES cell culture that includes protein components solely derived from recombinant sources or purified from human material. We describe the derivation of two new human ES cell lines in these defined culture conditions.
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