Human malaria parasites in continuous culture
W Trager, JB Jensen - Science, 1976 - science.org
W Trager, JB Jensen
Science, 1976•science.orgPlasmodium falciparum can now be maintained in continuous culture in human erythrocytes
incubated at 38° C in RPMI 1640 medium with human serum under an atmosphere with 7
percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material,
derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times
by the addition of human erythrocytes at 3-or 4-day intervals. The parasites continued to
reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly …
incubated at 38° C in RPMI 1640 medium with human serum under an atmosphere with 7
percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material,
derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times
by the addition of human erythrocytes at 3-or 4-day intervals. The parasites continued to
reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly …
Plasmodium falciparum can now be maintained in continuous culture in human erythrocytes incubated at 38°C in RPMI 1640 medium with human serum under an atmosphere with 7 percent carbon dioxide and low oxygen (1 or 5 percent). The original parasite material, derived from an infected Aotus trivirgatus monkey, was diluted more than 100 million times by the addition of human erythrocytes at 3- or 4-day intervals. The parasites continued to reproduce in their normal asexual cycle of approximately 48 hours but were no longer highly synchronous. They have remained infective to Aotus.
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