[HTML][HTML] Uncoupling of acetylation from phosphorylation regulates FoxO1 function independent of its subcellular localization
The activity of transcription factor FoxO1 is regulated by phosphorylation-dependent nuclear
exclusion and deacetylation-dependent nuclear retention. It is unclear whether and how
these two post-translational modifications affect each other. To answer this question, we
expressed FoxO1 cDNAs with combined mutations of phosphorylation and acetylation sites
in HEK-293 cells and analyzed their subcellular localization patterns. We show that
mutations mimicking the acetylated state (KQ series) render FoxO1 more sensitive to Akt …
exclusion and deacetylation-dependent nuclear retention. It is unclear whether and how
these two post-translational modifications affect each other. To answer this question, we
expressed FoxO1 cDNAs with combined mutations of phosphorylation and acetylation sites
in HEK-293 cells and analyzed their subcellular localization patterns. We show that
mutations mimicking the acetylated state (KQ series) render FoxO1 more sensitive to Akt …