[CITATION][C] The intercellular cement in pemphigus vulgaris, an electron microscopic study
K Hashimoto, WF Lever - Dermatology, 1967 - karger.com
K Hashimoto, WF Lever
Dermatology, 1967•karger.comMaterials and Methods Five oral and seven cutaneous lesions of pemphigus vulgaris were
studied. Small tissue blocks wrere immediately fixed in a 1% solution of osmic acid which
had been adjusted to pH 8.0 with veronal buffer. After one and a half hours' fixation, the
specimens were dehydrated and embedded in Araldite. Thin sections, cut on an LKB
Ultrotome, were stained with 1% uranyl acetate in 30% ethanol and with Reynold's lead
citrate solution (6). Sections thus prepared were examined in an RCA EMU-3G electron …
studied. Small tissue blocks wrere immediately fixed in a 1% solution of osmic acid which
had been adjusted to pH 8.0 with veronal buffer. After one and a half hours' fixation, the
specimens were dehydrated and embedded in Araldite. Thin sections, cut on an LKB
Ultrotome, were stained with 1% uranyl acetate in 30% ethanol and with Reynold's lead
citrate solution (6). Sections thus prepared were examined in an RCA EMU-3G electron …
Materials and Methods
Five oral and seven cutaneous lesions of pemphigus vulgaris were studied. Small tissue blocks wrere immediately fixed in a 1% solution of osmic acid which had been adjusted to pH 8.0 with veronal buffer. After one and a half hours’ fixation, the specimens were dehydrated and embedded in Araldite. Thin sections, cut on an LKB Ultrotome, were stained with 1% uranyl acetate in 30% ethanol and with Reynold’s lead citrate solution (6). Sections thus prepared were examined in an RCA EMU-3G electron microscope.
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