Micrometer scale Ex Vivo multiphoton imaging of unstained arterial wall structure
T Boulesteix, AM Pena, N Pagès… - Cytometry Part A: the …, 2006 - Wiley Online Library
T Boulesteix, AM Pena, N Pagès, G Godeau, MP Sauviat, E Beaurepaire…
Cytometry Part A: the journal of the International Society for …, 2006•Wiley Online LibraryBackground We characterize the application of multiphoton microscopy to the observation of
the extracellular matrix of fresh unstained vessels. Method Combined two‐photon‐excited
fluorescence (2PEF) and second harmonic generation (SHG) imaging of large arteries
reveals the architecture of elastin and collagen fibers in the vessel wall with remarkable
specificity. Results We present elastin/collagen imaging in unstained rat vessels at both
micrometer and whole vessel scales, and we characterize the optical properties of rat carotid …
the extracellular matrix of fresh unstained vessels. Method Combined two‐photon‐excited
fluorescence (2PEF) and second harmonic generation (SHG) imaging of large arteries
reveals the architecture of elastin and collagen fibers in the vessel wall with remarkable
specificity. Results We present elastin/collagen imaging in unstained rat vessels at both
micrometer and whole vessel scales, and we characterize the optical properties of rat carotid …
Background
We characterize the application of multiphoton microscopy to the observation of the extracellular matrix of fresh unstained vessels.
Method
Combined two‐photon‐excited fluorescence (2PEF) and second harmonic generation (SHG) imaging of large arteries reveals the architecture of elastin and collagen fibers in the vessel wall with remarkable specificity.
Results
We present elastin/collagen imaging in unstained rat vessels at both micrometer and whole vessel scales, and we characterize the optical properties of rat carotid artery and aorta walls. We apply this method to evidence deleterious effects of residual doses of a pesticide on the vessel wall.
Conclusion
This study illustrates the potential of 2PEF/SHG microscopy for pharmacological studies in unlabeled arteries. © 2005 Wiley‐Liss, Inc.
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