Breadth of human immunodeficiency virus-specific neutralizing activity in sera: clustering analysis and association with clinical variables

NA Doria-Rose, RM Klein, MG Daniels, S O'Dell… - Journal of …, 2010 - Am Soc Microbiol
NA Doria-Rose, RM Klein, MG Daniels, S O'Dell, M Nason, A Lapedes, T Bhattacharya
Journal of virology, 2010Am Soc Microbiol
Induction of antibodies that neutralize a broad range of human immunodeficiency virus type
1 (HIV-1) isolates is a major goal of vaccine development. To study natural examples of
broad neutralization, we analyzed sera from 103 HIV-1-infected subjects. Among progressor
patients, 20% of sera neutralized more than 75% of a panel of 20 diverse viral isolates. Little
activity was observed in sera from long-term nonprogressors (elite controllers). Breadth of
neutralization was correlated with viral load, but not with CD4 count, history of past …
Abstract
Induction of antibodies that neutralize a broad range of human immunodeficiency virus type 1 (HIV-1) isolates is a major goal of vaccine development. To study natural examples of broad neutralization, we analyzed sera from 103 HIV-1-infected subjects. Among progressor patients, 20% of sera neutralized more than 75% of a panel of 20 diverse viral isolates. Little activity was observed in sera from long-term nonprogressors (elite controllers). Breadth of neutralization was correlated with viral load, but not with CD4 count, history of past antiretroviral use, age, gender, race/ethnicity, or route of exposure. Clustering analysis of sera by a novel method identified a statistically robust subgrouping of sera that demonstrated broad and potent neutralization activity.
American Society for Microbiology