[HTML][HTML] Associations of B-and C-Raf with cholesterol, phosphatidylserine, and lipid second messengers: preferential binding of Raf to artificial lipid rafts
M Hekman, H Hamm, AV Villar, B Bader… - Journal of Biological …, 2002 - ASBMB
The serine/threonine kinase C-Raf is a key mediator in cellular signaling. Translocation of
Raf to membranes has been proposed to be facilitated by Ras proteins in their GTP-bound
state. In this study we provide evidence that both purified B-and C-Raf kinases possess
lipophilic properties and associate with phospholipid membranes. In the presence of
phosphatidylserine and lipid second messengers such as phosphatidic acid and ceramides
these associations were very specific with affinity constants (KD) in the range of 0.5–50 nm …
Raf to membranes has been proposed to be facilitated by Ras proteins in their GTP-bound
state. In this study we provide evidence that both purified B-and C-Raf kinases possess
lipophilic properties and associate with phospholipid membranes. In the presence of
phosphatidylserine and lipid second messengers such as phosphatidic acid and ceramides
these associations were very specific with affinity constants (KD) in the range of 0.5–50 nm …