Constitutive hepatic glucokinase activity db/db and ob/ob mice
TT Yen, NB Stamm - Biochimica et Biophysica Acta (BBA)-Enzymology, 1981 - Elsevier
The specific activity of hepatic glucokinase (ATP: d-glucose 6-phosphotransferase, EC 2.7.
1.2) in db/db mice and ob/ob mice was higher than in normal mice. All enzymes had a
similar K m and, thus, the difference in activity was not due to differences in the affinity of
enzyme molecules to substrates. Mixing liver extracts with high or low enzyme activities
yielded additive results, as expected, which ruled out the involvement of an inhibitor or
activator of the enzyme. Fasting normal mice of either strain for three days decreased …
1.2) in db/db mice and ob/ob mice was higher than in normal mice. All enzymes had a
similar K m and, thus, the difference in activity was not due to differences in the affinity of
enzyme molecules to substrates. Mixing liver extracts with high or low enzyme activities
yielded additive results, as expected, which ruled out the involvement of an inhibitor or
activator of the enzyme. Fasting normal mice of either strain for three days decreased …