[HTML][HTML] Targeted inhibition of interferon-γ-dependent intercellular adhesion molecule-1 (ICAM-1) expression using dominant-negative Stat1
MJ Walter, DC Look, RM Tidwell, WT Roswit… - Journal of Biological …, 1997 - ASBMB
A subset of epithelial immune-response genes (including intercellular adhesion molecule-1
(ICAM-1)) depends on an IFN-γ signal transduction pathway with the Stat1 transcription
factor as a critical intermediate. Excessive local activation of this pathway may lead to airway
inflammation, so we sought to selectively down-regulate the pathway using a dominant-
negative strategy for inhibition of epithelial Stat1 in a primary culture airway epithelial cell
model. Using a Stat1-deficient cell line, we demonstrated that transfection of wild-type Stat1 …
(ICAM-1)) depends on an IFN-γ signal transduction pathway with the Stat1 transcription
factor as a critical intermediate. Excessive local activation of this pathway may lead to airway
inflammation, so we sought to selectively down-regulate the pathway using a dominant-
negative strategy for inhibition of epithelial Stat1 in a primary culture airway epithelial cell
model. Using a Stat1-deficient cell line, we demonstrated that transfection of wild-type Stat1 …