[HTML][HTML] Dual roles for glucokinase in glucose homeostasis as determined by liver and pancreatic β cell-specific gene knock-outs using Cre recombinase

C Postic, M Shiota, KD Niswender, TL Jetton… - Journal of Biological …, 1999 - ASBMB
Glucokinase (GK) gene mutations cause diabetes mellitus in both humans and mouse
models, but the pathophysiological basis is only partially defined. We have used cre-
loxPtechnology in combination with gene targeting to perform global, β cell-, and hepatocyte-
specific gene knock-outs of this enzyme in mice. Gene targeting was used to create a triple-
loxed gk allele, which was converted by partial or total Cre-mediated recombination to a
conditional allele lacking neomycin resistance, or to a null allele, respectively. β cell-and …