The ubiquitin‐activating enzyme E1‐like protein in lung cancer cell lines

PMJ McLaughlin, W Helfrich, K Kok… - … journal of cancer, 2000 - Wiley Online Library
PMJ McLaughlin, W Helfrich, K Kok, M Mulder, SW Hu, MGL Brinker, MHJ Ruiters
International journal of cancer, 2000Wiley Online Library
The UBE1L gene isolated from the chromosome 3p21 region has an extremely reduced
level of mRNA in lung cancer. Sequence analysis showed a 45% homology to the human
ubiquitin‐activating enzyme E1 at the amino acid level. To further characterize the protein
product, we generated UBE1L protein‐specific antibodies. Immunoblot analysis revealed a
full‐length gene product of approximately 112 kDa. Assessment of the level and distribution
pattern of the UBE1L protein in normal and tumor tissue using the generated antibodies …
Abstract
The UBE1L gene isolated from the chromosome 3p21 region has an extremely reduced level of mRNA in lung cancer. Sequence analysis showed a 45% homology to the human ubiquitin‐activating enzyme E1 at the amino acid level. To further characterize the protein product, we generated UBE1L protein‐specific antibodies. Immunoblot analysis revealed a full‐length gene product of approximately 112 kDa. Assessment of the level and distribution pattern of the UBE1L protein in normal and tumor tissue using the generated antibodies showed that the UBE1L protein was present in normal lung cells and non‐lung cancer cell lines, but was undetectable in all 14 human lung cancer cell lines analyzed. This difference in expression of the UBE1L protein between normal lung tissue and lung tumor‐derived cell lines suggests a possible involvement of an E1‐like protein in the origin and/or progression of lung tumors. Int. J. Cancer 85:871–876, 2000. © 2000 Wiley‐Liss, Inc.
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