[HTML][HTML] High-throughput immunoblotting: ubiquitin-like protein ISG15 modifies key regulators of signal transduction

MP Malakhov, KI Kim, OA Malakhova… - Journal of Biological …, 2003 - ASBMB
ISG15 is a ubiquitin-like protein that conjugates to numerous proteins in cells treated with
interferon or lipopolysaccharide. Dysregulation of protein ISG15 modification (ISGylation) in
mice leads to decreased life expectancy, brain cell injury, and hypersensitivity to interferon.
Although ISG15 was identified more than two decades ago, the exact biochemical and
physiological functions of ISG15-modification remain unknown, and the proteins targeted by
ISG15 have not been identified. The major purpose of this work was to identify ISG15 targets …