Vector and parameters for targeted transgenic RNA interference in Drosophila melanogaster
JQ Ni, M Markstein, R Binari, B Pfeiffer, LP Liu… - Nature …, 2008 - nature.com
JQ Ni, M Markstein, R Binari, B Pfeiffer, LP Liu, C Villalta, M Booker, L Perkins, N Perrimon
Nature methods, 2008•nature.comThe conditional expression of hairpin constructs in Drosophila melanogaster has emerged
in recent years as a method of choice in functional genomic studies. To date, upstream
activating site–driven RNA interference constructs have been inserted into the genome
randomly using P-element–mediated transformation, which can result in false negatives due
to variable expression. To avoid this problem, we have developed a transgenic RNA
interference vector based on the phiC31 site-specific integration method.
in recent years as a method of choice in functional genomic studies. To date, upstream
activating site–driven RNA interference constructs have been inserted into the genome
randomly using P-element–mediated transformation, which can result in false negatives due
to variable expression. To avoid this problem, we have developed a transgenic RNA
interference vector based on the phiC31 site-specific integration method.
Abstract
The conditional expression of hairpin constructs in Drosophila melanogaster has emerged in recent years as a method of choice in functional genomic studies. To date, upstream activating site–driven RNA interference constructs have been inserted into the genome randomly using P-element–mediated transformation, which can result in false negatives due to variable expression. To avoid this problem, we have developed a transgenic RNA interference vector based on the phiC31 site-specific integration method.
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