Transient and Stable Transgenesis Using Tol2 Transposon Vectors

H Kikuta, K Kawakami - Zebrafish: Methods and protocols, 2009 - Springer
H Kikuta, K Kawakami
Zebrafish: Methods and protocols, 2009Springer
Transgenesis is an important methodology for studying the function of genes and genomes
in model plants and animals. For the model vertebrate zebrafish, methods using the Tol2
transposable element have been developed for this purpose. With these methods, the
function of the transgene can be analyzed in both transient and stable transgenic fish.
Recently, cis-sequences necessary for transposition of the Tol2 element were revealed. This
enabled development of transposon vectors containing minimal DNA sequences that are …
Summary
Transgenesis is an important methodology for studying the function of genes and genomes in model plants and animals. For the model vertebrate zebrafish, methods using the Tol2 transposable element have been developed for this purpose. With these methods, the function of the transgene can be analyzed in both transient and stable transgenic fish. Recently, cis-sequences necessary for transposition of the Tol2 element were revealed. This enabled development of transposon vectors containing minimal DNA sequences that are easily manipulated. More recently, several transposon vectors containing the Gateway sequence were created and reported. These are useful because any foreign sequences can be cloned into a transposon vector fairly easily and rapidly. This chapter describes the features of these transposon vectors, and protocols to perform transgenesis in zebrafish.
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