[HTML][HTML] Long exposure to high glucose concentration impairs the responsive expression of γ-glutamylcysteine synthetase by interleukin-1β and tumor necrosis factor …
Y Urata, H Yamamoto, S Goto, H Tsushima… - Journal of Biological …, 1996 - ASBMB
To elucidate the pathological metabolism of glutathione synthesis in diabetic endothelial
cells, we studied the expression of γ-glutamylcysteine synthetase (γ-GCS) using a mouse
vascular endothelial cell line. Exposing normoglycemic endothelial cells to tumor necrosis
factor-α (TNF-α) or interleukin-1β (IL-1β) increased the activity and the mRNA expression of
γ-GCS. The addition of inhibitors for nuclear factor κB (NF-κB) to the cells caused a loss of
the γ-GCS mRNA expression in response to TNF-α. A shift of the concentration of glucose in …
cells, we studied the expression of γ-glutamylcysteine synthetase (γ-GCS) using a mouse
vascular endothelial cell line. Exposing normoglycemic endothelial cells to tumor necrosis
factor-α (TNF-α) or interleukin-1β (IL-1β) increased the activity and the mRNA expression of
γ-GCS. The addition of inhibitors for nuclear factor κB (NF-κB) to the cells caused a loss of
the γ-GCS mRNA expression in response to TNF-α. A shift of the concentration of glucose in …