[HTML][HTML] Determination of substrate motifs for human Chk1 and hCds1/Chk2 by the oriented peptide library approach
T O'Neill, L Giarratani, P Chen, L Iyer, CH Lee… - Journal of Biological …, 2002 - ASBMB
Mammalian Chk1 and Chk2 are two Ser/Thr effector kinases that play critical roles in DNA
damage-activated cell cycle checkpoint signaling pathways downstream of ataxia
telangiectasia-mutated and ataxia telangiectasia-related. Endogenous substrates have
been identified for human hCds1/Chk2 and Chk1; however, the sequences surrounding the
substrate residues appear unrelated, and consensus substrate motifs for the two Ser/Thr
kinases remain unknown. We have utilized peptide library analyses to develop specific …
damage-activated cell cycle checkpoint signaling pathways downstream of ataxia
telangiectasia-mutated and ataxia telangiectasia-related. Endogenous substrates have
been identified for human hCds1/Chk2 and Chk1; however, the sequences surrounding the
substrate residues appear unrelated, and consensus substrate motifs for the two Ser/Thr
kinases remain unknown. We have utilized peptide library analyses to develop specific …