Peptidomics-based approach reveals the secretion of the 29-residue COOH-terminal fragment of the putative tumor suppressor protein DMBT1 from pancreatic …

K Sasaki, K Sato, Y Akiyama, K Yanagihara, M Oka… - Cancer research, 2002 - AACR
K Sasaki, K Sato, Y Akiyama, K Yanagihara, M Oka, K Yamaguchi
Cancer research, 2002AACR
Deleted in malignant brain tumors 1 is a putative tumor suppressor protein in brain, lung,
esophageal, gastric, and colorectal cancer. Here we report the mass spectrometric
identification of a 3335 Da peptide, which was found in serum-free conditioned medium from
5 of 15 pancreatic adenocarcinoma cell lines but not from 35 carcinoma cell lines and 2
nonmalignant pancreatic duct cell lines. The peptide was the 29 COOH-terminal amino
acids from deleted in malignant brain tumors 1. It is suggested that the peptide is generated …
Abstract
Deleted in malignant brain tumors 1 is a putative tumor suppressor protein in brain, lung, esophageal,gastric, and colorectal cancer. Here we report the mass spectrometric identification of a 3335 Da peptide, which was found in serum-free conditioned medium from 5 of 15 pancreatic adenocarcinoma cell lines but not from 35 carcinoma cell lines and 2 nonmalignant pancreatic duct cell lines. The peptide was the 29 COOH-terminal amino acids from deleted in malignant brain tumors 1. It is suggested that the peptide is generated inside the cells by limited proteolysis and extracellularly secreted. Our peptidomics-based approach will help screen candidate marker peptides for a particular type of cancer.
AACR