[HTML][HTML] Tenascin-C aptamers are generated using tumor cells and purified protein
BJ Hicke, C Marion, YF Chang, T Gould… - Journal of Biological …, 2001 - ASBMB
Tenascin-C (TN-C) is an extracellular matrix protein that is overexpressed during tissue
remodeling processes, including tumor growth. To identify an aptamer for testing as a tumor-
selective ligand, SELEX (systematicevolution of ligands by exponential enrichment)
procedures were performed using both TN-C and TN-C-expressing U251 glioblastoma cells.
The different selection techniques yielded TN-C aptamers that are related in sequence. In
addition, a crossover procedure that switched from tumor cell to purified protein selections …
remodeling processes, including tumor growth. To identify an aptamer for testing as a tumor-
selective ligand, SELEX (systematicevolution of ligands by exponential enrichment)
procedures were performed using both TN-C and TN-C-expressing U251 glioblastoma cells.
The different selection techniques yielded TN-C aptamers that are related in sequence. In
addition, a crossover procedure that switched from tumor cell to purified protein selections …