[HTML][HTML] Biomarker discovery from pancreatic cancer secretome using a differential proteomic approach* S
M Grønborg, TZ Kristiansen, A Iwahori, R Chang… - Molecular & Cellular …, 2006 - ASBMB
Quantitative proteomics can be used as a screening tool for identification of differentially
expressed proteins as potential biomarkers for cancers. Candidate biomarkers from such
studies can subsequently be tested using other techniques for use in early detection of
cancers. Here we demonstrate the use of stable isotope labeling with amino acids in cell
culture (SILAC) method to compare the secreted proteins (secretome) from pancreatic
cancer-derived cells with that from non-neoplastic pancreatic ductal cells. We identified 145 …
expressed proteins as potential biomarkers for cancers. Candidate biomarkers from such
studies can subsequently be tested using other techniques for use in early detection of
cancers. Here we demonstrate the use of stable isotope labeling with amino acids in cell
culture (SILAC) method to compare the secreted proteins (secretome) from pancreatic
cancer-derived cells with that from non-neoplastic pancreatic ductal cells. We identified 145 …