Structural differences between insulin receptors in the brain and peripheral target tissues.
KA Heidenreich, NR Zahniser, P Berhanu… - Journal of Biological …, 1983 - ASBMB
Insulin receptors in various brain regions (olfactory tubercle, hippocampus, and
hypothalamus) were photoaffinity labeled using the photoreactive analogue of insulin B2 (2-
nitro, 4-azidophenylacetyl)-des-PheB1-insulin (NAPA-DP-insulin). A protein with an
apparent Mr of 400,000 was specifically labeled with 125I-NAPA-DP-insulin in all three brain
regions. When radiolabeled proteins were reduced with dithiothreitol prior to
electrophoresis, specific labeling occurred predominantly in a protein with an apparent Mr of …
hypothalamus) were photoaffinity labeled using the photoreactive analogue of insulin B2 (2-
nitro, 4-azidophenylacetyl)-des-PheB1-insulin (NAPA-DP-insulin). A protein with an
apparent Mr of 400,000 was specifically labeled with 125I-NAPA-DP-insulin in all three brain
regions. When radiolabeled proteins were reduced with dithiothreitol prior to
electrophoresis, specific labeling occurred predominantly in a protein with an apparent Mr of …