A novel method to quantify sphingosine 1-phosphate by immobilized metal affinity chromatography (IMAC)
Sphingosine 1-phosphate (S1P), a lysophospholipid mediator that signals through G protein-
coupled receptors, regulates a wide plethora of biological responses such as angiogenesis
and immune cell trafficking. Detection and quantification of S1P in biological samples is
challenging due to its unique physicochemical nature and occurrence in trace quantities. In
this report, we describe a new method to selectively enrich S1P and dihydro-S1P from
biological samples by the Fe3+ gel immobilized metal affinity chromatography (IMAC). The …
coupled receptors, regulates a wide plethora of biological responses such as angiogenesis
and immune cell trafficking. Detection and quantification of S1P in biological samples is
challenging due to its unique physicochemical nature and occurrence in trace quantities. In
this report, we describe a new method to selectively enrich S1P and dihydro-S1P from
biological samples by the Fe3+ gel immobilized metal affinity chromatography (IMAC). The …