Epigenetics of multiple myeloma after treatment with cytostatics and gamma radiation
J Krejčí, A Harničarová, D Štreitová, R Hájek, L Pour… - Leukemia research, 2009 - Elsevier
Genetic and epigenetic changes in multiple myeloma (MM) correlate with the stage of the
disease. Therefore, we investigated how cytostatics and gamma radiation influence MM-
associated histone modifications. ChIP-PCR and ChIP-on-chip technologies were used to
quantify H3K9 acetylation and H3K9 dimethylation at select loci in MM patients,
lymphoblastoid ARH-77, and myeloma MOLP-8 cells. Genome-wide analysis revealed that
the cytostatic, melphalan, increased H3K9 acetylation at multiple gene promoters in ARH-77 …
disease. Therefore, we investigated how cytostatics and gamma radiation influence MM-
associated histone modifications. ChIP-PCR and ChIP-on-chip technologies were used to
quantify H3K9 acetylation and H3K9 dimethylation at select loci in MM patients,
lymphoblastoid ARH-77, and myeloma MOLP-8 cells. Genome-wide analysis revealed that
the cytostatic, melphalan, increased H3K9 acetylation at multiple gene promoters in ARH-77 …