purpose. To determine the baseline characteristics, reliability, and dynamic range of the pattern electroretinogram (PERG) as a tool to monitor progressive RGC dysfunction in the DBA/2J mouse model of glaucoma with spontaneously elevated intraocular pressure (IOP).

methods. PERGs were recorded from 56 undilated eyes of 28 anesthetized (ketamine-xylazine-acepromazine) DBA/2J mice of different ages (2–4 months, n= 44 eyes; 12–14 months, n= 12 eyes) in response to contrast reversal of gratings that maximize PERG amplitude (95% contrast, 1-Hz reversal, 0.05 cyc/deg spatial frequency, 50× 56 field size). Robust averaging (1800 sweeps) was used to isolate PERG from background noise. Cone-driven ERGs in response to diffuse light flashes superimposed on a rod-adapting background (FERG) were also recorded.

results. PERGs had consistent waveforms and were reproducible across batches of mice and operators. In 2-to 4-month-old mice (prehypertensive stage), the PERG amplitude (mean, 8.15±0.4 μV [SEM]) was considerably larger than the noise (mean 1.18±0.1 μV). The test–retest variability (two different sessions 1 week apart) and interocular asymmetry of PERG amplitude was approximately 30%, and that of PERG latency was approximately 17%. In 12-to 14-month-old mice (advanced hypertensive stage) the PERG amplitude (mean, 1.29±0.12 μV) was close to that of noise. In 12-to 14-month-old mice the FERG was reduced to a lesser extent compared with the PERG.

conclusions. The PERG has an adequate signal-to-noise ratio, reproducibility, and dynamic range to monitor the progression of functional changes in the inner retina in DBA/2J mice.