[HTML][HTML] Models of the cooperative mechanism for Rho effector recognition: implications for RhoA-mediated effector activation
L Blumenstein, MR Ahmadian - Journal of Biological Chemistry, 2004 - ASBMB
Activated GTPases of the Rho family regulate a spectrum of functionally diverse downstream
effectors, initiating a network of signal transduction pathways by interaction and activation of
effector proteins. Although effectors are defined as proteins that selectively bind the GTP-
bound state of the small GTPases, there have been also several indications for a nucleotide-
independent binding mode. By characterizing the molecular mechanism of RhoA interaction
with its effectors, we have determined the equilibrium dissociation constants of several Rho …
effectors, initiating a network of signal transduction pathways by interaction and activation of
effector proteins. Although effectors are defined as proteins that selectively bind the GTP-
bound state of the small GTPases, there have been also several indications for a nucleotide-
independent binding mode. By characterizing the molecular mechanism of RhoA interaction
with its effectors, we have determined the equilibrium dissociation constants of several Rho …