Thiazolidinediones and glucocorticoids synergistically induce differentiation of human adipose tissue stromal cells: biochemical, cellular, and molecular analysis
YDC Halvorsen, A Bond, A Sen… - Metabolism …, 2001 - metabolismjournal.com
While adipocyte differentiation has been studied extensively in murine cultures, the lack of a
readily available preadipocyte model has hindered equivalent studies in man. We describe
methods for the isolation and culture of primary human stromal cells from surgical adipose
tissue specimens. In vitro, the stromal cells rapidly differentiate in response to a combination
of adipogenic agents. Among these, glucocorticoids and thiazolidinediones act together to
induce the formation of lipid vacuoles within the cells. These morphologic changes …
readily available preadipocyte model has hindered equivalent studies in man. We describe
methods for the isolation and culture of primary human stromal cells from surgical adipose
tissue specimens. In vitro, the stromal cells rapidly differentiate in response to a combination
of adipogenic agents. Among these, glucocorticoids and thiazolidinediones act together to
induce the formation of lipid vacuoles within the cells. These morphologic changes …