A novel multiparametric flow cytometry-based cytotoxicity assay simultaneously immunophenotypes effector cells: comparisons to a 4 h 51Cr-release assay
GG Kim, VS Donnenberg, AD Donnenberg… - Journal of …, 2007 - Elsevier
Natural killer (NK) cell-or T cell-mediated cytotoxicity traditionally is measured in 4–16 h
51Cr-release assays (CRA). A new four-color flow cytometry-based cytotoxicity assay (FCC)
was developed to simultaneously measure NK cell cytotoxicity and NK cell phenotype (CD3-
CD16+ CD56+). Target cells, K562 or Daudi, were labeled with Cell Tracker Orange (CTO)
prior to the addition of effector cells. Following co-incubation, 7 amino-actinomycin D (7-
AAD) was added to measure death of target cells. The phenotype of effectors, viability of …
51Cr-release assays (CRA). A new four-color flow cytometry-based cytotoxicity assay (FCC)
was developed to simultaneously measure NK cell cytotoxicity and NK cell phenotype (CD3-
CD16+ CD56+). Target cells, K562 or Daudi, were labeled with Cell Tracker Orange (CTO)
prior to the addition of effector cells. Following co-incubation, 7 amino-actinomycin D (7-
AAD) was added to measure death of target cells. The phenotype of effectors, viability of …