Myelination in mouse dorsal root ganglion/Schwann cell cocultures
S Päiväläinen, M Nissinen, H Honkanen, O Lahti… - Molecular and Cellular …, 2008 - Elsevier
The established protocols for in vitro studies of peripheral nerve myelination with rat
embryonic dorsal root ganglia (DRG) and postnatal Schwann cell cocultures do not work
with mouse cells. Consequently, the full potential of this model, which would allow to perform
cell type-specific, mixed genotype cocultures without cross-breeding the animals, cannot be
exploited. We determined the conditions required to promote full myelination in cocultures of
pre-purified mouse embryonic DRG and neonatal Schwann cells, and present a method …
embryonic dorsal root ganglia (DRG) and postnatal Schwann cell cocultures do not work
with mouse cells. Consequently, the full potential of this model, which would allow to perform
cell type-specific, mixed genotype cocultures without cross-breeding the animals, cannot be
exploited. We determined the conditions required to promote full myelination in cocultures of
pre-purified mouse embryonic DRG and neonatal Schwann cells, and present a method …