[HTML][HTML] Bile salt–dependent lipase interacts with platelet CXCR4 and modulates thrombus formation in mice and humans

L Panicot-Dubois, GM Thomas… - The Journal of …, 2007 - Am Soc Clin Investig
L Panicot-Dubois, GM Thomas, BC Furie, B Furie, D Lombardo, C Dubois
The Journal of clinical investigation, 2007Am Soc Clin Investig
Bile salt–dependent lipase (BSDL) is an enzyme involved in the duodenal hydrolysis and
absorption of cholesteryl esters. Although some BSDL is transported to blood, the role of
circulating BSDL is unknown. Here, we demonstrate that BSDL is stored in platelets and
released upon platelet activation. Because BSDL contains a region that is structurally
homologous to the V3 loop of HIV-1, which binds to CXC chemokine receptor 4 (CXCR4),
we hypothesized that BSDL might bind to CXCR4 present on platelets. In human platelets in …
Bile salt–dependent lipase (BSDL) is an enzyme involved in the duodenal hydrolysis and absorption of cholesteryl esters. Although some BSDL is transported to blood, the role of circulating BSDL is unknown. Here, we demonstrate that BSDL is stored in platelets and released upon platelet activation. Because BSDL contains a region that is structurally homologous to the V3 loop of HIV-1, which binds to CXC chemokine receptor 4 (CXCR4), we hypothesized that BSDL might bind to CXCR4 present on platelets. In human platelets in vitro, both BSDL and a peptide corresponding to its V3-like loop induced calcium mobilization and enhanced thrombin-mediated platelet aggregation, spreading, and activated αIIbβ3 levels. These effects were abolished by CXCR4 inhibition. BSDL also increased the production of prostacyclin by human endothelial cells. In a mouse thrombosis model, BSDL accumulated at sites of vessel wall injury. When CXCR4 was antagonized, the accumulation of BSDL was inhibited and thrombus size was reduced. In BSDL–/– mice, calcium mobilization in platelets and thrombus formation were attenuated and tail bleeding times were increased in comparison with those of wild-type mice. We conclude that BSDL plays a role in optimal platelet activation and thrombus formation by interacting with CXCR4 on platelets.
The Journal of Clinical Investigation