Replication-defective adenovirus vectors with multiple deletions do not induce measurable vector-specific T cells in human trials

RA Koup, L Lamoreaux, D Zarkowsky, RT Bailer… - Journal of …, 2009 - Am Soc Microbiol
RA Koup, L Lamoreaux, D Zarkowsky, RT Bailer, CR King, JGD Gall, DE Brough…
Journal of virology, 2009Am Soc Microbiol
The magnitude and character of adenovirus serotype 5 (Ad5)-specific T cells were
determined in volunteers with and without preexisting neutralizing antibodies (NAs) to Ad5
who received replication-defective Ad5 (rAd5)-based human immunodeficiency virus
vaccines. There was no correlation between T-cell responses and NAs to Ad5. There was no
increase in magnitude or activation state of Ad5-specific CD4+ T cells at time points where
antibodies to Ad5 and T-cell responses to the recombinant gene products could be …
Abstract
The magnitude and character of adenovirus serotype 5 (Ad5)-specific T cells were determined in volunteers with and without preexisting neutralizing antibodies (NAs) to Ad5 who received replication-defective Ad5 (rAd5)-based human immunodeficiency virus vaccines. There was no correlation between T-cell responses and NAs to Ad5. There was no increase in magnitude or activation state of Ad5-specific CD4+ T cells at time points where antibodies to Ad5 and T-cell responses to the recombinant gene products could be measured. These data indicate that rAd5-based vaccines containing deletions in the E1, E3, and E4 regions do not induce appreciable expansion of vector-specific CD4+ T cells.
American Society for Microbiology