[CITATION][C] Human T cell proliferative response to polypeptides from adenovirus type 2

BE Souberbielle, WC Russell - Journal of Infectious Diseases, 1995 - academic.oup.com
BE Souberbielle, WC Russell
Journal of Infectious Diseases, 1995academic.oup.com
3. Clerici M, Levin JM, Kessler HA, et al. HlV-specific T-helper activity in seronegative health
care workers exposed to contaminated blood. JAMA 1994; 271: 42-6. used three antigenic
systems for standardization purposes: Mycobacterium tuberculosis, tetanus toxin, and Ad-2.
In our hands, the nitrocellulose T cell technique was reproducible only when modifications
described by Lee et al.[3] and some of our own were followed. In brief, the critical step for the
reproducibility of the technique was the resuspension of the NCP particles, previously …
3. Clerici M, Levin JM, Kessler HA, et al. HlV-specific T-helper activity in seronegative health care workers exposed to contaminated blood. JAMA 1994; 271: 42-6. used three antigenic systems for standardization purposes: Mycobacterium tuberculosis, tetanus toxin, and Ad-2. In our hands, the nitrocellulose T cell technique was reproducible only when modifications described by Lee et al.[3] and some of our own were followed. In brief, the critical step for the reproducibility of the technique was the resuspension of the NCP particles, previously dissolved in DMSO, in a bicarbonate buffer at constant and slow speed using a peristaltic pump [4]. We tested 1 healthy individual (HLA-A2,-All;-B44,-B55;-C4,-C6;-DR3,-DR5) for possible T cell response against separated Ad-2 polypeptides hexon (H), penton (P), fiber (F), V, VI, and VII. The Ad-2 virions were prepared and purified using standard techniques and gave the well-established polypeptide pattern on SDS-PAGE [5]. Significant proliferative response, using the microparticle technique, was seen mainly against the F and VI polypeptides and to a lesser extent against the H polypeptide (figure 1). No significant proliferative response over background was seen against the P, V, and VII polypeptides. The same subject's peripheral blood mononuclear cells proliferated well (almost as much as against tetanus toxin) when incubated for 7 days with purified Ad-2 virions.
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