RESULTS

The effect of 8 X lo-* M oxamate on the time course of growth and glycolysis of HeLa 22a cells, over a period of 220 hours is shown in Fig. 1. The concentration of oxamate used has been found in previous experiments to result in 90 to 95% inhibition of glycolysis by Ehrlich ascites tumor cells, in vitro (1). These data show that in the presence of 8 X 1O-2 M oxamate the growth of HeLa 22a cells is completely inhibited. In fact, there is a significant drop in protein from the initial value of 78 pg of total protein to a constant value of approximately 40 pg of total protein. Microscopic examination of these cultures revealed that there were some cells which remained attached to the glass surface at the end of the experiment. These cells appeared unchanged morphologically. a 80 k 40 I-