Modification of primary and recurrent genital herpes in guinea pigs by passive immunization

N Bourne, RB Pyles, DI Bernstein… - Journal of General …, 2002 - microbiologyresearch.org
N Bourne, RB Pyles, DI Bernstein, LR Stanberry
Journal of General Virology, 2002microbiologyresearch.org
Guinea pigs were administered antiserum 24 h (As+ 24) or 72 h (As+ 72) after intravaginal
herpes simplex virus type 2 (HSV-2) challenge. Treatment at either time reduced acute virus
replication in the dorsal root ganglia and the overall magnitude of replication in the genital
tract. In two studies, As+ 24 treatment significantly reduced the severity of primary genital
skin disease and the frequency of subsequent spontaneous recurrent disease. In contrast,
As+ 72 treatment produced a modest reduction in primary disease severity but did not …
Guinea pigs were administered antiserum 24 h (As+24) or 72 h (As+72) after intravaginal herpes simplex virus type 2 (HSV-2) challenge. Treatment at either time reduced acute virus replication in the dorsal root ganglia and the overall magnitude of replication in the genital tract. In two studies, As+24 treatment significantly reduced the severity of primary genital skin disease and the frequency of subsequent spontaneous recurrent disease. In contrast, As+72 treatment produced a modest reduction in primary disease severity but did not impact on recurrent disease. Quantitative PCR analysis of dorsal root ganglia DNA from latently infected animals showed that As+24 treatment produced a significantly reduced viral DNA burden, which appeared to correlate with the reduction in recurrent disease. The amount of DNA in the ganglia of As+72-treated animals was not significantly lower than that of controls. These observations have implications for both the dynamics of latency establishment and desirable vaccine characteristics.
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