Optimized large-scale production of high titer lentivirus vector pseudotypes
M Sena-Esteves, JC Tebbets, S Steffens… - Journal of virological …, 2004 - Elsevier
The goal of the present study was to develop an efficient transient transfection method for
large-scale production of high titer lentivirus vector stocks of eight different pseudotypes. The
envelope genes used for this purpose were those from VSV-G, Mokola, Rabies, MLV-
Ampho, MLV-10A1, LCMV-WE, and LCMV-Arm53b. All envelopes were cloned into phCMV,
which yielded lentivirus vector titers one, two, or three orders of magnitude higher than the
original plasmids for the Rabies, MLV-10A1, and MLV-Ampho envelopes, respectively …
large-scale production of high titer lentivirus vector stocks of eight different pseudotypes. The
envelope genes used for this purpose were those from VSV-G, Mokola, Rabies, MLV-
Ampho, MLV-10A1, LCMV-WE, and LCMV-Arm53b. All envelopes were cloned into phCMV,
which yielded lentivirus vector titers one, two, or three orders of magnitude higher than the
original plasmids for the Rabies, MLV-10A1, and MLV-Ampho envelopes, respectively …